Integrated transcriptome and metabolome analysis revealed the low pressure regulation in Saussurea involucrata leaves

The purified cDNA then underwent end repair, the incorporation of A tails, and ligation to sequencing junctions. This was succeeded by fragment size selection via DNA purification magnetic beads, and ultimately, PCR enrichment was conducted to generate the final cDNA libraries. These libraries were sequenced using the Illumina platform, resulting in a total of 6 cDNA libraries.