Barley apical spikelet abortion transcriptome

In barley (Hordeum vulgare), the indeterminate inflorescence meristem produces spikelet primordia along its axis and aborts a few terminally produced ones. In order to study the transcriptional changes involved in apical abortion of barley inflorescence, tissue-specific RNA-seq was performed at three early developmental stages by dividing the spikes into three parts, viz., apical, central, and basal. In two-rowed barley cv. Bowman, spikes were sampled at W5.0, W6.5, and W7.5 stages, whereas W5.5, W6.5 and W8.25 stages were collected from six-rowed barley cv. Morex. Three biological replicates were generated from each tissue type resulting in 54 RNA-seq samples.