Gene expression of APJ deficient endothelial cell

Molecular pathways regulating the development of arterial and venous endothelial cells (ECs) are now well-established, but control of parallel arterial-venous (A-V) alignment is unclear. We report that arterial-venous alignment in the skin is determined by apelin receptor (APJ) expression in venous ECs. We used microarrays to detail the global programme of gene expression in endothelial cells that has relationship with the deficient of APJ. Endothelial cells were marked and isolated by Fluorescence-activated cell sorting in Trizol.调控动脉与静脉内皮细胞（endothelial cells, ECs）发育的分子通路目前已得到充分阐明，但平行动静脉（arterial-venous, A-V）排列的调控机制仍不明确。本研究发现，皮肤中的动静脉排列由静脉内皮细胞中的Apelin受体（apelin receptor, APJ）的表达所决定。我们通过基因芯片（microarrays）详细解析了与Apelin受体缺陷相关的内皮细胞的全基因表达谱。实验中，我们通过荧光激活细胞分选术（Fluorescence-activated cell sorting）标记并分离了TRIzol试剂（Trizol）中的内皮细胞。