Macrophage Populations Isolated From Sham and Saline Intratracheally Instilled Mice: Maturity and Phenotype Data

Resident and recruited macrophages were identified via a chimeric mouse model. The macrophage populations of these chimeras were also assessed following sham and saline intratracheal instillation. Alveolar macrophages were collected via bronchoalveolar lavage and protein expression measured by flow cytometry. Macrophages were assessed for maturity and phenotype.

本研究通过嵌合小鼠模型（chimeric mouse model）鉴定了驻留型与招募型巨噬细胞。同时，本研究还对经假手术及生理盐水气管内滴注处理的上述嵌合小鼠体内的巨噬细胞群进行了评估。研究人员通过支气管肺泡灌洗（bronchoalveolar lavage）收集肺泡巨噬细胞，并采用流式细胞术（flow cytometry）检测其蛋白质表达水平。此外，本研究还对巨噬细胞的成熟状态与表型开展了分析评估。