Effect of cell density on HaCaT keratinocyte gene expression

Cell density affects keratinocyte behaviors and gene expression. Here, We examines the differential expressed genes regulated by cell density. We performed RNA-seq analysis to compare HaCaT cells cultured under high- or low0density conditions. The Gene Ontology Biological Process terms enriched among differential expressed genes through DESeq2 included cell adhesion and keratinocyte differentiation. Our data provides the understanding of keratinocyte behavior regulated by cell density. HaCaT cells were seeded into 6-cm plastic dishes at the concentration of 2.5 x 105/mL or 7.5 x 105/mL. After the overnight incubation at 37ºC in humidified 5% CO2, RNA was extracted.