Resting and activating NK cells_gene signatures. Homo sapiens

Genome-wide transcriptional profiling was used to identify gene expression signatures and pathways in resting and IL2 activated NK cell isolated from peripheral blood of healthy donors Keywords: Time series_NK cells gene profile Overall design: The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity (Figure1). Equivalent amount of total RNA from NK cells derived from 3 or 4 different donors for each time point was pooled, amplified and labeled before duplicate hybridizations was performed on spotted (pre-synthesized oligonucleotide) microarrays.