Assessment of fish communities using eDNA: effect of spatial sampling design in lentic systems of different sizes

01 Rawdata.xlsx Summary of sequence abundance data generated by the ECOTAG program. Table shows sequence abundance data of samples from Weiming Lake (SL), Kunming Lake (ML) and Guanting Reservoir (LL) generated by the ECOTAG program, retaining low-frequency sequences and negative control sequencing results, including the sample blank (SB), filtration blank (FB), extraction blank (EB), and PCR blank (PB). Also included are: total read counts of each sequence, taxid, taxonomic assignment by the ECOTAG program, taxonomic assignment at the species level by BLAST against GenBank nucleotide databases, the identity scores and accession numbers of the highest matched sequences, and the query sequences. Sequences with an assigned taxon of “NA” and human sequences were removed from the table. 02 Tele02_ngsfilter.txt File used to assign sequences to samples in the OBITOOLS package. File used to assign sequences to each sample in the OBITOOLS package, including the Teleo2 primers and primer tags. 03 Tele02_database.fa Reference database constructed by in silico PCR with the Teleo2 primers using the ECOPCR program. File shows the in silico PCR results using the Teleo2 primers to extract sequences in the EMBL database (release 138), which was used as the reference database in the ECOTAG program to assign a taxon to each sequence.

01 Rawdata.xlsx：本文件为ECOTAG程序生成的序列丰度数据汇总表。该表包含由ECOTAG程序生成的未名湖（SL）、昆明湖（ML）以及官厅水库（LL）的样本序列丰度数据，保留了低频序列与阴性对照测序结果，其中阴性对照包括样本空白（SB）、过滤空白（FB）、提取空白（EB）以及PCR空白（PB）。同时包含以下内容：每条序列的总读长计数、分类单元标识符（taxid）、ECOTAG程序给出的分类注释、通过BLAST比对GenBank核苷酸数据库得到的物种级分类注释、最高匹配序列的相似度得分与登录号，以及查询序列。表中已移除分类注释为"NA"的序列以及人类序列。
02 Tele02_ngsfilter.txt：本文件属于OBITOOLS软件包，用于将序列分配至各样本，其中包含Teleo2引物与引物标签。
03 Tele02_database.fa：本文件为通过ECOPCR程序使用Teleo2引物进行电子PCR（in silico PCR）构建的参考数据库。该文件展示了使用Teleo2引物从EMBL数据库（第138版）中提取序列的电子PCR结果，该数据库被用作ECOTAG程序中为每条序列赋予分类注释的参考数据库。