TGFbeta Induction in Tumors

TGFb1 has both tumor suppressive and pro-oncogenic functions in cancer pathogenesis but the underlying basis of this not well understood. We have developed a transgenic conditional epidermal expression model for TGFb1 in order to examine its immediate effects in benign and malignant chemically induced squamous cancers. The microarray study is an attempt to elucidate those genetic changes contributing to this behavior. Keywords: compound treatment design The experiments revolve around mouse transgenics. Therefore, the hybridization design does as well. Each array represents a bigenic chemically derived tumor (exogenous TGFb expressing)versus a monogenic tumor (no exogenous TGFb expression). Tumors were separated into Papillomas ad Squamous Cell Carcinomas. In total 14 papillomas and 9 carcinomas were included. 5 reverse fluors and 5 replicates were included.