Table 2 in Revisiting the taxonomy of Dioclea and related genera (Leguminosae, Papilionoideae), with new generic circumscriptions

Table 2. Sequences of the primers used for PCR amplification and sequencing, as well as PCR conditions.
DNA regionPrimer namePrimer Sequence 5 ’-3’ReferencePCR ConditionsPre-meltingDenaturation (I)Primer Annealing (II)Primer Extension (III)Cycles (I + II + III)Final ExtensionETS18S-IGSGAGACAAGCATATGACTACTGGCAGGATCAACCAGBaldwin and Markos (1998)94 °C (3 min)94 °C (1 min)55 °C (1 min)72 °C (1.5 min)3072 °C (7 min)ETS-DioGCTTGTGCATCGAACGGTTGGQueiroz et al. (2015)ITS17SE (F)ACGAATTCATGGTCCGGTGAAGTGTTCGSun et al. (1994)94 °C (3 min)94 °C (1 min)52 °C (40 s)72 °C (2.5 min)2872 °C (7 min)26SE (R)TAGAATTCCCCGGTTCGCTCGCCGTTACSun et al. (1994)5.8SACGACTCTCGGCAACSun et al. (1994)5.8RGCGTGACGCCCAGGCSun et al. (1994)SSFGTCGTAACAAGGTTTCCGTAGKollipara et al. (1997)Following manufacturer’s protocol for sequencingLSRGTTAGTTTCTTTTCCTCCKollipara et al. (1997)trnK/ matKmatK685FGTATCGCACTATGTATTATTTGAWojciechowski et al. (2004)94 °C (3 min)94 °C (40 s)55 °C (45 s)72 °C (1 min)3672 °C (7 min)matK4LaCCTTCGATACTGGGTGAAAGATWojciechowski et al. (2004)matK1100LTTCAGTGGTACGGAGTCAAATGWojciechowski et al. (2004)matK4RCATCTTTCACCCAGTAGCGAAGHu et al. (2000)matK1932RCAGACCGGCTTACTAATGGGHu et al. (2000)trnK2RCCCGGAACTAGTCGGATGWojciechowski et al. (2004)