Inner filter effect correction for fluorescence measurements in microplates (AddAbs) - experimental data
收藏资源简介：
Experimental data for the paper entitled <em>Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber</em> (https://doi.org/10.1021/acs.analchem.3c01295). Separate worksheets are provided for the following: 1. Fluorescence measurements - raw values in triplicate; 2 worksheets for transparent (T) and nontransparent (NT) microplates, each with 15 titrations: L<sub>1</sub>-L<sub>12</sub> and H<sub>1</sub>-H<sub>3</sub>, 2. Absorbance measurements - raw values in triplicate (<em>λ</em><sub>ex</sub> = 345 nm, <em>λ</em><sub>em</sub> = 390 nm); 1 worksheet for T microplates only, with 4 titrations: L<sub>1</sub>-L<sub>4</sub>, 3. Fluorescence measurements - averaged, baseline-corrected averaged and normalized baseline-corrected averaged triplicates; 2 worksheets for T and NT microplates, each with 15 titrations: L<sub>1</sub>-L<sub>12</sub> and H<sub>1</sub>-H<sub>3</sub>, 4. ZINFE/NINFE-corrected fluorescence in T microplates; 12 worksheets for 12 titrations: L<sub>1</sub>-L<sub>9</sub> and H<sub>1</sub>-H<sub>3</sub>, 5. ZINFE/NINFE-corrected fluorescence in NT microplates; 12 worksheets for 12 titrations: L<sub>1</sub>-L<sub>9</sub> and H<sub>1</sub>-H<sub>3</sub>. The results of the ZINFE and NINFE correction methods obtained using the online calculator service written in Javascript: https://ninfe.science (version 09.5.2022.) For additional details please visit: https://glymech.pharma.hr//GlyMech.html.
