Identification and characterisation of immunoreactive fungal proteins recognised by sera from Zimbabweans sensitised to fungi

Exposure and sensitisation to fungal allergens poses a serious threat to human health. The prevalence of fungal allergic disease is increasing globally but is poorly studied in Africa. This study aimed to identify and characterise fungal proteins that were immunoreactive against serum samples from fungal-sensitised Zimbabweans. A pool of eight sera (3-5 years old) from fungi (Aspergillus fumigatus, Alternaria alternata, Cladosporium herbarum, Epicoccum nigrum, Penicillium chrysogenum, Rhizopus nigricans and Saccharomyces cerevisiae) sensitive Zimbabwean children (as determined by Skin prick tests) was used to screen proteins for immunoreactivity. Protein bands recognised by the sera were identified via mass spectrometry and bioinformatic analysis. The data on the identified proteins and the metadata of the eight children are presented here.

真菌变应原暴露与致敏对人类健康构成严重威胁。全球范围内真菌变应性疾病的患病率呈持续上升趋势，但非洲地区对此类疾病的研究仍较为匮乏。本研究旨在鉴定并表征可与真菌致敏的津巴布韦人群血清样本发生免疫反应的真菌蛋白。本研究采用8份经皮肤点刺试验（Skin prick test）确认的、对烟曲霉（Aspergillus fumigatus）、链格孢（Alternaria alternata）、草部枝孢霉（Cladosporium herbarum）、黑附球菌（Epicoccum nigrum）、产黄青霉（Penicillium chrysogenum）、黑根霉（Rhizopus nigricans）及酿酒酵母（Saccharomyces cerevisiae）致敏的津巴布韦儿童血清样本（样本保存时长为3至5年），用于筛选具有免疫反应性的真菌蛋白。通过质谱分析与生物信息学分析，对可被该血清识别的蛋白条带进行了鉴定。本数据集现已公开本次研究鉴定得到的蛋白相关数据，以及这8名受试儿童的元数据。