Additional file 1: of Swift metabolite changes and leaf shedding are milestones in the acclimation process of grapevine under prolonged water stress

Table S1. Stomatal conductance measurements of 2014 and 2015 presented as mean (N = 4) and standard error. The respective time points are indicated with days from treatment application. Table S2. Fold change (Treatment/Control) of leaf secondary metabolites during the time course of the experiment in 2014 and 2015. Table S3. Fold change (Treatment/Control) of leaf central metabolites during the time course of the experiment in 2014 and 2015. Table S4. Summary of RNA sequencing analysis metrics. The table show the number of filtered reads and aligned reads (filtered) to the 12X grapevine reference genome that are counted. Table S5. Transcript abundance of the all 29,970 genes, reported as Reads Per Kilobase of transcript, per Million mapped reads (RPKM), in leaves of well-watered and water deficit treated grapevines at day 4, 8 (2014) and day 12 (2015). Tables S6–S8. Summary of differentially expressed genes (FDR 1) in grapevine leaves comparing well-watered and water deficit treatments. DESeq2 statistical outputs of day 4, 8 (2014) and day 12 (2015) treatments are listed in Table S6, S7, and S8, respectively.. Annotation of genes were referred from Vitisnet ( https://www.sdstate.edu/agronomy-horticulture-and-plant-science/functional-genomics-bud-endodormancy-induction-grapevines-5 ) (Grimplet et al. 2009). Table S9. List of differentially expressed genes in grapevine leaves that are common and unique leaves in each intersection of the Venn diagram described in Fig. 4b. Table S10. Summary of enriched (FDR

表S1：2014年与2015年的气孔导度测定结果，以平均值（N=4）及标准误形式呈现，相应时间点以处理施加后的天数标注。
表S2：2014年与2015年实验周期内，叶片次生代谢物的倍数变化值（处理组/对照组）。
表S3：2014年与2015年实验周期内，叶片中心代谢物的倍数变化值（处理组/对照组）。
表S4：RNA测序（RNA sequencing）分析指标汇总。该表格展示了过滤后读数以及与12X葡萄参考基因组比对的过滤后读数的计数情况。
表S5：29970个全部基因的转录本丰度，以每百万比对读数每千碱基转录本读数（Reads Per Kilobase of transcript, per Million mapped reads，RPKM）表示，样本来自水分充足与水分胁迫处理的葡萄叶片，采集时间为第4天、第8天（2014年）及第12天（2015年）。
表S6~S8：对比水分充足与水分胁迫处理的葡萄叶片中差异表达基因（错误发现率False Discovery Rate，FDR 1）的汇总结果。对应第4天（2014年）、第8天（2014年）及第12天（2015年）处理组的DESeq2统计输出分别列于表S6、S7、S8。基因注释参考自Vitisnet（https://www.sdstate.edu/agronomy-horticulture-and-plant-science/functional-genomics-bud-endodormancy-induction-grapevines-5）（Grimplet等，2009）。
表S9：图4b中维恩图（Venn diagram）各交集内共有的及特有的葡萄叶片差异表达基因列表。
表S10：富集分析结果汇总（FDR ）