Dataset for paper 'Highly Selective and Rapidly Activatable Fluorogenic Thrombin Sensors and Application in Human Lung Tissue'

Dataset related to publication DOI: 10.1039/c7ob00663b. A library of FRET-based peptides were prepared and studied as Thrombin substrates. This identified probes that showed selective activation by Thrombin, low background signal, stability to Factor Xa, matrix metalloproteases, and primary human inflammatory cell lysates and supernatant. These were selected for further optimization, creating a second generation of fluorogenic probes with improved solubility and Plasmin resistance. The optimized probe detected Thrombin activity ex vivo in fibrotic human lung tissue.

本数据集关联发表于DOI: 10.1039/c7ob00663b的研究工作。研究人员构建并表征了一类基于荧光共振能量转移（FRET）的肽库，并将其作为凝血酶（Thrombin）底物开展研究。经筛选得到了可被凝血酶选择性激活、背景信号低，且对凝血因子Xa（Factor Xa）、基质金属蛋白酶以及原代人炎症细胞裂解液与上清液具有良好稳定性的探针分子。随后选取上述探针进行后续优化，获得了第二代荧光探针，其溶解性与抗纤溶酶（Plasmin）能力均得到显著提升。经优化后的探针可在离体实验中检测纤维化人肺组织内的凝血酶活性。