Expression data from Inflammatory breast cancer (IBC) cells

ERβ expression is associated with less metastasis in patients with IBC tumors. We investigated this association in preclinical models of IBC by knocking out ERβ in cells. Ablation of ERβ promotes migration and invasion of IBC cells and increases the metastatic potential of IBC tumors in vivo. We used microarrays to detail the global programme of gene expression underlying the increased migration of ERβ knockout cells and identified distinct classes of up-regulated genes during this process. Inflammatory breast cancer KPL4 cells with CRISPR/CAS9-mediated knockout of ERβ were used for RNA extraction and hybridization on Affymetrix microarrays