Global heart transcript data from fasted male BXD strains on chow or high fat diet

Transcript data from heart tissue from fasted-state male BXD strains on chow or high fat diet We used microarrays to compare the heart expression differences across the BXD strain family and across two diverse diets 29-week-old male mice were fasted overnight (6pm-9am), anesthetized under isoflurane, and perfused, then hearts were snap-frozen in liquid nitrogen for RNA extraction and RNEasy cleanup. Each dietary and strain cohort consisted of ~5 animals which were prepared independently then pooled evenly by ~5 µg RNA before the Affymetrix arrays were run.本数据集为普通饲料或高脂饮食条件下禁食状态雄性BXD品系小鼠的心脏组织转录组数据。本研究利用基因芯片技术，对比分析BXD品系小鼠家族在两种差异化饮食方案下的心脏基因表达差异。实验中，29周龄雄性小鼠于夜间禁食15小时（下午6时至次日上午9时），经异氟烷麻醉后实施心脏灌流，随后将心脏组织置于液氮中快速冷冻，用于后续RNA提取及RNeasy纯化。每个饮食-品系分组包含约5只独立制备的小鼠，在开展Affymetrix基因芯片检测前，将各组小鼠的RNA以约5μg的量均匀混合后上机检测。