Copper mesocosm 16S communities

Outdoor freshwater mesocosms spiked with varying concentrations of copper were sampled in order to profile microbial communities. Samples collected include water, sediment and cellulose baits. DNA was extracted from all sample types. In addition to this, RNA was extracted from sediment samples and reverse transcribed to get cDNA. The v4 region of the 16S ribosomal RNA gene was amplified using Earth Microbiome primers 515F and 806R.

The 16S rRNA gene amplicons were sequenced on the Illumina MiSeq platform with 250 base pair-end reads. Forward and reverse read alignments, quality filtering, chimera checking and OTU clustering were performed using USEARCH v9.

OTU sequences were submitted to GenBank, however, 130 were found to be too novel for submission and thus are made available here.

本研究针对添加了梯度浓度铜离子的室外淡水中型生态系统（mesocosms）开展采样，以解析其微生物群落组成。采集样本涵盖水体、沉积物与纤维素诱饵三类。所有样本均完成脱氧核糖核酸（DNA）提取；此外，另从沉积物样本中提取核糖核酸（RNA），并通过反转录获得互补脱氧核糖核酸（cDNA）。采用地球微生物组计划（Earth Microbiome Project）的引物515F与806R，对16S核糖体RNA（rRNA）基因的V4可变区进行扩增。
16S rRNA基因扩增子在Illumina MiSeq平台上进行250碱基对双端测序。借助USEARCH v9软件完成双端读段比对、质量过滤、嵌合体检测与操作分类单元（OTU）聚类分析。
所有操作分类单元序列已提交至GenBank，但其中130条序列因过于新颖无法完成提交，故在此公开。