Richard Allen (University of Hawaii) (2011) CIL:13131, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

The cytoproct is the site of spent vacuole defecation on the surface of Paramecium. When closed the cytoproct forms a long narrow wavy ridge along the posterior suture. At its apex the ridge is about 0.2µm wide and it can be as much as 12µm or more in length. This ridge has the ability to be pulled down against the approaching spent vacuole where the plasma membrane covering the midline of the ridge will meet and fuse with the membrane of the vacuole. The ridge contains unique fibers on both of its lips that mesh with each other as they meet inside the ridge (see Allen and Wolf, J. Cell Sci. 14:611-631, 1974). TEM taken on 4/19/79 by R. Allen with Hitachi HU11A operating at 60kV. Neg. 10,000X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12343) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

胞质囊是草履虫表面排空泡的排泄场所。当闭合时，胞质囊沿后缝合线形成一条细长波状的脊。在该脊的顶端，其宽度约为0.2微米，长度可达12微米或更长。此脊具有被拉向即将到达的排空泡的能力，在此过程中，覆盖脊中线之血浆膜的边缘将与泡膜相遇并融合。脊的两唇含有独特的纤维，当它们在脊内相遇时相互交织（参见Allen和Wolf，J. Cell Sci. 14:611-631，1974）。1979年4月19日，R. Allen使用60kV的日立HU11A电子显微镜拍摄了透射电子显微镜图像。负片放大10,000倍。刻度尺长度为0.5微米。负片印在纸上，图像被扫描到Photoshop中。此数字化图像可用于定性分析。未经处理的、高分辨率的图像版本（CIL:12343）存档于图书馆，可供定量分析。使用标准戊二醛固定，随后用四氧化锇处理，用酒精脱水，并嵌入环氧树脂中。使用微切机制备的切片厚度约为75纳米。更多信息请参考（http://www5.pbrc.hawaii.edu/allen/）。