Plant Gravity Perception: Pilot (PGP Pilot)

This study examined tissue-specific early transcriptional responses upon reorientation in wild type and starchless pgm-1 mutant seedlings of Arabidopsis. Seedlings were grown in vertical Petri dishes for four days. Treatment samples were reoriented 90 degrees for ten minutes while control plants remained vertical. All seedlings were flash frozen in liquid nitrogen and RNA was extracted from root tips, mature root, hypocotyl, and cotyledon fractions of the seedlings. RNA was sequenced via NovaSeq.

本研究探究了野生型与无淀粉型pgm-1突变体（starchless pgm-1 mutant）拟南芥（Arabidopsis）幼苗在重定向后的组织特异性早期转录响应。将幼苗置于垂直培养皿中培养四天；处理组样本进行90度重定向处理十分钟，对照组植株则保持垂直状态。所有幼苗均经液氮快速冷冻，随后从幼苗的根尖、成熟根、下胚轴及子叶组分中提取RNA，并采用NovaSeq平台完成RNA测序。