A study of SMCs phenotype in TGFβR2iSMC-Apoe and TGFβR2iSMC-Ldlr mice Chen et al. Related to Fig 2

Data Related to Fig 2. A mouse line carrying floxed Tgfbr2 and SMC lineage-tracing mT/mG alleles under control of a myosin heavy chain (Myh11) promoter on an Ldlr null background (Ldlr-/-;Myh11CreERT2;mT/mGf/f;Tgfbr2f/f) hereafter called TGFβR2iSMC-Ldlr. These mice were treated with Tamoxifen at 6 week of age and fed high cholesterol high fat diet for 4 months. Ascending aorta smooth muscle cells from these mice express macrophage, chondrocyte, adipocyte, and osteoblast lineage markers as shown by immunocytochemistry and qRT-PCT from laser micro dissection tissues compared to controls.

图2相关数据集。该小鼠品系携带loxP侧翼Tgfbr2（转化生长因子β受体2）等位基因与受肌球蛋白重链（Myh11）启动子调控的平滑肌细胞（smooth muscle cell, SMC）谱系示踪mT/mG等位基因，且处于低密度脂蛋白受体（Ldlr）敲除背景（基因型为Ldlr-/-;Myh11CreERT2;mT/mGf/f;Tgfbr2f/f），后续将其命名为TGFβR2iSMC-Ldlr。该品系小鼠于6周龄时接受他莫昔芬诱导，并饲喂高胆固醇高脂饲料4个月。与对照组相比，经激光显微切割获取的该小鼠升主动脉平滑肌细胞，通过免疫细胞化学染色及qRT-PCT检测证实，可表达巨噬细胞、软骨细胞、脂肪细胞及成骨细胞谱系标志物。