Neurochemistry, morphology and projection patterns of myenteric nerve cell bodies, traced from circular muscle and myenteric plexus of guinea pig distal colon

Raw mapping data describing the locations of retrogradely labelled myenteric nerve cell bodies traced from a single location in the myenteric plexus or from circular muscle. Each row represents a single myenteric nerve cell body. X and Y coordinates provide the longitudinal and circumferential distance of the nerve cell body in millimetres, relative to 0, respectively. The DiI-application site in each preparation is designated (0, 0). Colonic preparations were oriented such that negative X values represent locations oral to the DiI application site (0, 0). Thus, nerve cell bodies whose X coordinate is a negative value is located oral to the DiI-application site and therefore has a descending axonal projection; the opposite is true for positive values. The dataset comprises 9 experiments/preparations derived from 9 animals. Five preparations, coded CM3-7, were used to trace nerve cell bodies from circular muscle. Four preparations, coded MP2-5, were used to trace nerve cell bodies from the myenteric plexus. The column “target” divides these two types of preparations into “0” circular muscle and “1” myenteric plexus. The column “NOS” indicates whether the nerve cell body contained immunohistochemically-detectable nitric oxide synthase, whereby “0” indicates a lack of immunoreactive content and “1” indicates the presence of immunoreactive content. Nerve cell bodies were similarly classified for “CALR”, calretinin, and “CALB”, calbindin. “BASK”, indicates whether the nerve cell body was located inside dense clusters of calbindin-immunoreactive varicosities, whereby “0” was negative and “1” was positive for this condition. The column “morph” indicates whether the myenteric nerve cell body had Dogiel type 1 or type 2 mophological characteristics, coded “1” and “2”, respectively.Nerve cell bodies in preparation MP4 lack circumferential data. These data were intentionally omitted as nerve cell bodies in this preparation were traced from 3 circumferentially-aligned DiI-application sites. A single DiI-application site was used in all other preparationsDate coverage: 2018

本数据集为原始映射数据，描述了从肌间丛（myenteric plexus）单一位置或环层肌（circular muscle）逆行标记的肌间神经胞体的位置。每一行对应单个肌间神经胞体。X、Y坐标分别代表神经胞体相对于原点(0, 0)的纵向与周向距离，单位为毫米。每个标本的DiI标记位点记为(0, 0)。结肠标本的定向规则为：X负值表示位于DiI标记位点(0, 0)的口侧。因此，X坐标为负值的神经胞体位于DiI标记位点口侧，其轴突为下行投射；正值则反之。

本数据集包含来自9只实验动物的9份标本。其中5份编号为CM3-7的标本用于追踪环层肌来源的神经胞体，4份编号为MP2-5的标本用于追踪肌间丛来源的神经胞体。字段“target”将两类标本划分为“0”（环层肌）与“1”（肌间丛）。

字段“NOS”（一氧化氮合酶，nitric oxide synthase）用于标记神经胞体是否含有免疫组化可检测的一氧化氮合酶，其中“0”代表无免疫反应性，“1”代表存在免疫反应性。字段“CALR”（钙视网膜蛋白，calretinin）与“CALB”（钙结合蛋白，calbindin）则依据两类钙结合蛋白的免疫反应性对神经胞体进行分类。“BASK”用于标记神经胞体是否位于钙结合蛋白免疫反应性曲张体的致密簇内，其中“0”代表阴性结果，“1”代表阳性结果。

字段“morph”用于标注肌间神经胞体是否具有Dogiel I型（Dogiel type 1）或Dogiel II型（Dogiel type 2）形态学特征，分别编码为“1”与“2”。

标本MP4的周向数据存在缺失，该数据被有意省略，原因是此标本中的神经胞体来自3个沿周向排列的DiI标记位点，其余所有标本均仅使用单个DiI标记位点。

数据覆盖年份：2018年