Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats [unfractionated PB]

Naked mole-rats are a mammalian model organism of exceptional longevity. We mapped the hematopoietic hierarchy and used functional characterizations to define a purified stem and progenitor cell (HSPC) compartment similar to mouse LIN-/Sca-1+/Kit+ HSPCs or human LIN-/CD34+/CD38lo HSPCs. Naked mole-rat peripheral blood was extracted, RBC-lysed and submitted to scRNA-Seq as unfractionated BM. The mRNA libraries were aligned to either the FRAMA bone marrow transcriptome assembly from our publication. Note: Raw data represents only gene expresion (GEX). Raw data for ADT not included.