Supplementary Material for: The Human IgE Repertoire

IgE is a key mediator in allergic diseases. However, in strong contrast to other antibody isotypes, many details of the composition of the human IgE repertoire are poorly defined. The low levels of human IgE in the circulation and the rarity of IgE-producing B cells are important reasons for this lack of knowledge. In this review, we summarize the current knowledge on these repertoires both in terms of their complexity and activity, i.e. knowledge which despite the difficulties encountered when studying the molecular details of human IgE has been acquired in recent years. We also take a look at likely future developments, for instance through improvements in sequencing technology and methodology that allow the isolation of additional allergen-specific human antibodies mimicking IgE, as this certainly will support our understanding of human IgE in the context of human disease in the years to come.

IgE是过敏性疾病的关键介导因子。然而，与其他抗体同种型（antibody isotypes）形成鲜明对比，人类IgE免疫组库（repertoire）的组成细节仍有诸多尚未被清晰阐明之处。循环中人类IgE含量极低，且产IgE B细胞（IgE-producing B cells）数量极为稀少，这是当前对其认知不足的重要原因。在本综述中，我们将梳理当前关于这类免疫组库复杂性与功能活性的已有研究进展——尽管在解析人类IgE的分子细节时面临诸多挑战，但近年来相关研究已积累了丰硕成果。我们还将展望未来的研究前景，例如通过优化测序技术与实验方法，实现更多模拟IgE特性的变应原特异性（allergen-specific）人类抗体的分离，这无疑将助力我们在未来数年中深化对人类疾病背景下IgE的认知。