The molecular cytogenetic characterization of Conopophaga lineata indicates a common chromosome rearrangement in the Parvorder Furnariida (Aves, Passeriformes)

Abstract Cytogenetic analyses of the Suboscines species are still scarce, and so far, there is no karyotype description of any species belonging to the family Conopophagidae. Thus, the aim of this study is to describe and analyze the karyotype of Conopophaga lineata by chromosome painting using Gallus gallus (GGA) probes and to identify the location of the 18/28S rDNA cluster. Metaphases were obtained from fibroblast culture from two individuals of C. lineata. We observed a diploid number of 2n=78. GGA probes showed that most ancestral syntenies are conserved, except for the fission of GGA1 and GGA2, into two distinct pairs each. We identified the location of 18S rDNA genes in a pair of microchromosomes. The fission of the syntenic group corresponding to GGA2 was observed in other Furnariida, and hence may correspond to a chromosomal synapomorphy for the species of Parvorder Furnariida.

霸鹟亚目（Suboscines）物种的细胞遗传学分析仍较为匮乏，截至目前尚无隶属于食蚊鸟科（Conopophagidae）的任何物种的核型描述。本研究旨在利用原鸡（Gallus gallus, GGA）染色体涂染探针对棕食蚊鸟（Conopophaga lineata）的核型进行描述与分析，并定位18/28S核糖体DNA（rDNA）簇的位置。本研究从2只棕食蚊鸟个体的成纤维细胞培养物中获取了分裂中期染色体相，观察到其二倍体染色体数目为2n=78。原鸡染色体涂染探针结果显示，除原鸡1号染色体（GGA1）与原鸡2号染色体（GGA2）各自断裂为两个独立的染色体对之外，多数祖先保守同线性连锁群均得以保留。本研究在一对微型染色体上定位到了18S rDNA基因的位点。对应原鸡2号染色体的连锁群断裂现象此前已在其他灶鸟小目（Furnariida）类群中被观测到，因此该特征可能属于灶鸟小目（Parvorder Furnariida）物种的染色体共有衍征。