Chromatin immunoprecipitation (ChIP)-sequencing of Arabidopsis plants in response to Spodoptera litura attack

Histone modification is a cellular process for transcriptional regulation. In herbivore-damaged plants, activation of genes involved in defense responses is required for antiherbivore properties, but little is known about how the chromatin remodeling system is involved. In this study, our primary objective was to understand the epigenetic transcriptional regulatory system in Arabidopsis plants in response to damage by larvae of Spodoptera litura, a generalist herbivore. We performed chromatin immunoprecipitation (ChIP) sequencing to identify genes with enriched histone acetylation in leaves of Arabidopsis thaliana wild-type and a T-DNA insertion mutant line of HAC1, a histone acetyltransferase, and a transgenic line of HDA6, a histone deacetylase, in response to 24 h attack by S. litura larvae.