Gregory Antipa (San Francisco State University) (2011) CIL:17451, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

A transverse section near the mid region of the oral proboscis of Didinium. This micrograph shows the extrusive organelles of the mucocysts adjacent to the plasma membrane and the toxicysts. The pexicysts reside in the central region of the proboscis anterior to this section. A section toward the tip of the proboscis is found at (CIL:17452). Further details available at Wessenberg, H. and Antipa, G. 1968. Studies on Didinium nasutum. I. Structure and ultrastructure. Protistologica 4:427-447. And details of the operation of this structure is available at Wessenberg, H. and Antipa, G. 1970. Capture and ingestion of Paramecium by Didinium nasutum. J. Protozool. 17:250-270. TEM taken in 1966 by G. Antipa with JEM T6S operating at 50kV. Neg. 6,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 50nm thickness.

在Didinium口吻中部区域附近的横切面。该显微照片展示了相邻于质膜和毒囊的粘液囊外分泌器官。皮克囊位于该横切面前方口吻的中央区域。口吻尖端的切片可参见(CIL:17452)。更多细节可参考Wessenberg, H. 和 Antipa, G. 1968年的研究《关于Didinium nasutum的研究。I. 结构与超微结构》。该结构的运作细节可在Wessenberg, H. 和 Antipa, G. 1970年的论文《Didinium nasutum对Paramecium的捕食与摄取》中找到。1966年由G. Antipa使用50kV运行的JEM T6S透射电子显微镜拍摄。负片放大倍数为6000倍。原始胶片使用Epson Perfection V750 Pro扫描。此图像可供定量分析。采用标准戊二醛固定，随后用四氧化锇处理，用酒精脱水，并嵌入环氧树脂中。采用约50纳米厚度的切片由微刀切割制备。