MOESM4 of Comparative analysis on the expression of L1 loci using various RNA-Seq preparations

Additional file 4. The number of uniquely mapped upstream reads up to 1000 and 5000 bps upstream aligned with the manually curated, strand-specific, whole-cell, RNA-Seq data from replicate 1. In the first column are the L1 locus ID numbers, in the second column are the number of sense reads that map uniquely to the specific L1, in the third column is the reason for acceptance or rejection as authentically expressed L1 s, in the fourth column are the number of sense reads uniquely mapping up to 1000 bps upstream the specific L1, and in the fifth column are the number of sense reads uniquely mapping up to 5000 bps upstream the specific L1. In green are the L1 s curated to be expressed off their own promoters. In red are the L1 s curated to be passively transcribed off a promoter unrelated to the L1.

附加文件4。本文件统计了经与重复实验1的人工注释链特异性全细胞RNA测序（RNA-Seq）数据比对后，各L1元件上游1000 bp及5000 bp范围内的唯一比对上游读数。表格各列说明如下：第一列为L1基因座ID编号；第二列为唯一匹配至对应L1元件的有义读数数量；第三列为该L1元件被认定为真实表达或予以排除的判定依据；第四列为唯一匹配至对应L1元件上游1000 bp区间内的有义读数数量；第五列为唯一匹配至对应L1元件上游5000 bp区间内的有义读数数量。其中，以绿色标记的为经人工注释确认可通过自身启动子启动表达的L1元件；以红色标记的为经人工注释确认可通过与L1元件无关的启动子发生被动转录的L1元件。