Single cell RNA sequencing of LSK (lineage negative, Sca-1+, c-Kit+) cells isolated by fluorescence-activated cell sorting from the bone marrow of control young C57Bl/6Jax mice

Hematopoietic stem cells (HSCs) and different multipotent progenitor populations (MPPs) contained in the Lin- Sca-1+ c-Kit+ (LSK) compartment have previously been identified using diverse surface marker panels. However, a comprehensive and complete characterization of the complete LSK compartment is lacking to date. Here, we present a single cell RNA sequencing of LSK cells that was used to establish trajectories within the HSPC compartment. A related bulk 3' seq study of HSCs and MPP1-4 cells was also conducted, available in the ArrayExpress database under accession number E-MTAB-7391. Another related 3'-seq study of MPP5 population is available in the ArrayExpress database under accession number E-MTAB-9135.此前，研究人员已通过多种表面标志物组合，鉴定出造血干细胞（Hematopoietic stem cells, HSCs）以及Lin⁻ Sca-1⁺ c-Kit⁺（LSK）群体中包含的各类多能祖细胞群（multipotent progenitor populations, MPPs）。然而截至目前，针对完整LSK群体的全面且完整的特征解析仍存在空白。本研究对LSK细胞开展单细胞RNA测序，以此构建造血干祖细胞区室内的细胞分化轨迹。此外，本研究还同步开展了一项针对HSCs及MPP1-4细胞的批量3'端测序相关研究，相关数据已上传至ArrayExpress数据库，登录号为E-MTAB-7391。另有一项针对MPP5群体的3'端测序相关研究，其数据同样收录于ArrayExpress数据库，登录号为E-MTAB-9135。