Micropropagation of Physalis species with economic potential

ABSTRACT The objective of this work was to evaluate asepsis protocols, composition and concentration of culture media in the in vitro establishment of three physalis species. In experiment I, seeds of 3 species were used x 3 asepsis protocols. In experiment II, 3 culture media and explants 3 species were used. In experiment III, explants of 2 species x 4 concentrations of MS culture medium were used. In experiment IV, explants of 2 species x 4 sucrose concentrations were used. For experiments II, III and IV after 30 days were evaluated some phytotechnical parameters. The experimental design was completely randomized, in a 3 x 3 (experiments I and II) and 2 x 4 (experiments III and IV) factorial scheme. Protocols II and III were appropriate for P. peruviana germination, and I and III for P. minima, the three protocols were efficient for controlling fungi and bacteria. The MS is the most suitable for the in vitro establishment of three species. The culture medium at 100% concentration obtained better values in the in vitro development parameters evaluated for the species P. peruviana and P. minima. Sucrose concentrations close to 50 and 20 g L-1 favored the establishment of P. peruviana and P. minima.

摘要 本研究旨在评估3种酸浆属（Physalis）植物体外培养建立体系中的无菌操作方案、培养基组成及浓度。试验一以3个物种的种子为材料，设置3种无菌操作方案开展实验；试验二以3种培养基与3个物种的外植体（explant）为研究材料开展实验；试验三以2个物种的外植体与4个浓度梯度的MS培养基（Murashige & Skoog medium）为材料进行实验；试验四以2个物种的外植体与4个蔗糖浓度梯度为材料开展实验。针对试验二、三、四，于接种培养30天后对若干植物栽培相关参数进行测定与评价。本研究采用完全随机设计，其中试验一、二采用3×3因子设计，试验三、四采用2×4因子设计。研究结果显示，无菌方案二与三适用于毛酸浆（P. peruviana）的种子萌发，方案一与三适用于小酸浆（P. minima）；三种无菌方案均能有效防控真菌与细菌污染。MS培养基是3种酸浆属植物体外培养建立体系的最适培养基。针对毛酸浆与小酸浆，100%浓度的MS培养基在测定的体外发育参数中均获得最优值。当蔗糖浓度分别接近50 g·L⁻¹与20 g·L⁻¹时，分别有利于毛酸浆与小酸浆的体外培养建立。