Anti-hyperlipidemic and anti-diabetic evaluation of ethanolic leaf extract of Catharanthus roseus alone and in combination therapy

Abstract The use of plants in disease treatment is cost effective and relatively safe. This study was designed to investigate anti-hyperlipidemic and anti-diabetic activity of ethanolic leaf extract of Catharanthus roseus alone and in combination therapy in hyperlipidemic & diabetic mice. Eight groups comprising five mice each were used. Group A was hyperlipidemic control, group B, C, D received atorvastatin (20 mg/kg), leaf extract (200 mg/kg) and leaf extract in combination with atorvastatin (200 mg/kg and 20 mg/kg) orally for 15 days. Group E was diabetic control. Group F, G, H received sitagliptin (40 mg/kg), leaf extract (200 mg/kg) and extract in combination with sitagliptin (200 mg/kg and 40 mg/kg) orally for 7 days. Blood cholesterol levels were measured at 1st, 5th, 10th and 15th day and fasting blood sugar levels were measured at 2, 12, 24, 72 and 168 hours during treatment. One-way ANOVA with tukey- kramer multiple comparison test was used. The chemical characterization of ethanolic extract of Catharanthus roseus leaves showed presence of alkaloids, saponins, tannins and flavonoids. Ethanolic extract of Catharanthus roseus has significant anti-hyperlipidemic & anti-diabetic effects (p<0.05, p<0.01) when compared with control but had not cause significantly increase in anti-hyperlipidemic effects of atorvastatin. While significantly increased the antidiabetic effect of sitagliptin (p<0.05).

摘要：植物用于疾病治疗兼具成本效益与相对安全性。本研究旨在探究长春花（Catharanthus roseus）叶乙醇提取物单独给药及联合用药方案，对高脂血症与糖尿病小鼠的抗高脂血症及抗糖尿病活性。实验共设置8组小鼠，每组5只。对照组A为高脂血症模型组；B、C、D组分别灌胃给予阿托伐他汀（Atorvastatin，20 mg/kg）、长春花叶乙醇提取物（200 mg/kg）、提取物与阿托伐他汀联合给药（200 mg/kg提取物+20 mg/kg阿托伐他汀），连续给药15天。对照组E为糖尿病模型组；F、G、H组分别灌胃给予西他列汀（Sitagliptin，40 mg/kg）、长春花叶乙醇提取物（200 mg/kg）、提取物与西他列汀联合给药（200 mg/kg提取物+40 mg/kg西他列汀），连续给药7天。分别于给药第1、5、10、15天检测血清胆固醇水平，于给药期间的2、12、24、72及168小时检测空腹血糖水平。数据分析采用单因素方差分析（One-way ANOVA）结合Tukey-Kramer多重比较检验。长春花叶乙醇提取物的化学表征结果显示，其含有生物碱、皂苷、鞣质与黄酮类成分。与对照组相比，长春花叶乙醇提取物具有显著的抗高脂血症与抗糖尿病活性（p<0.05，p<0.01），但未显著增强阿托伐他汀的抗高脂血症作用；然而其可显著增强西他列汀的抗糖尿病活性（p<0.05）。