Data for "Sacchride Contents Change and the Role of PI3K-Akt Signal Transduction Pathway in Silkworm Eggs Treated with HCl to Prevent or Break Diapause"

Acid soaking with HCl is a common way to prevent or break diapause of silkworm eggs and has been widely used in production. However, its mechanism remains exploring. Few literature reported in metabolism of mono-, or disaccharides, and the differential expression patterns of genes and proteins related to the PI3K/Akt signaling pathway across the stages of silkworm diapause eggs, non-diapause eggs, instant acid soaking eggs, and red bean color acid-soaked eggs (Rbcase). Silkworm 932 strains eggs at different stages were used to explore the contents of several saccharides and analyze the expression profiles of 4 diapause-related genes (BmAkt, BmGsk-3, BmGs, and CaMKII) in the PI3K-Akt signaling pathway via qPCR. The results showed that in addition to monosaccharides such as glucose and fructose, the contents of polysaccharides, maltose, trehalose, and sorbitol varied significantly at different stages and states of the eggs. The expression profiles of the 4 genes were differently low but increased in instant acid-soaked eggs and Rbcase before hatching. Western blotting results showed two bands at 51 kDa and 61 kDa for BmAkt. The ratio of p-Akt/Akt in diapause eggs and Rbcase was equivalent to each other but significantly higher in Rbcase after activation of PI3K-Akt signaling pathway. These findings revealed that saccharides are basic energic sources, and PI3K-Akt signaling pathway play important roles in acid soaking to prevent or break diapause eggs.

盐酸（HCl）浸渍是当前防控或打破家蚕卵滞育的常用技术手段，已在蚕业生产中得到广泛应用。然而其具体作用机制仍有待深入阐释。目前鲜有文献聚焦于单糖、双糖的代谢过程，以及家蚕滞育卵、非滞育卵、即时盐酸浸渍卵与红豆色酸浸卵（Rbcase）各发育阶段中PI3K/Akt信号通路（PI3K/Akt signaling pathway）相关基因与蛋白的差异表达模式。本研究以家蚕932品系不同发育阶段的卵为实验材料，采用qPCR技术检测PI3K-Akt信号通路中4个滞育相关基因（BmAkt、BmGsk-3、BmGs及CaMKII）的表达谱，并定量分析多种糖类物质的含量。结果显示，除葡萄糖、果糖等单糖外，多糖、麦芽糖、海藻糖及山梨醇的含量在家蚕卵的不同发育阶段与生理状态下均存在显著差异。4个目标基因的基础表达水平普遍较低，但在即时盐酸浸渍卵与红豆色酸浸卵孵化前均出现上调趋势。蛋白质免疫印迹（Western blotting）结果显示，BmAkt可检测到51 kDa与61 kDa两条特异性条带。在PI3K-Akt信号通路激活后，滞育卵与红豆色酸浸卵的p-Akt/Akt比值基本持平，但红豆色酸浸卵的该比值显著更高。本研究结果表明，糖类物质作为家蚕卵发育的核心能量来源，PI3K-Akt信号通路在盐酸浸渍防控或打破家蚕卵滞育的过程中发挥了关键调控作用。