Pathogenic diversity of rice yellow mottle virus isolates from western Burkina Faso (2015-2021): phenotypic evaluation data

The rice yellow mottle virus (RYMV) constitutes a major threat to the actual development of rice cultivation in Africa. As climate change and agricultural intensification increase the risk of epidemics, there is an urgent need to understand the spatio-temporal dynamics of RYMV at local scale. This dataset provides information on the pathogenicity of RYMV isolates collected in western Burkina Faso between 2015 and 2021. Two experiments were conducted in controlled greenhouse conditions (IRD Montpellier, France, 2021-2022), to characterize the phenotype of viral isolates from the major viral genetic groups identified in western Burkina Faso, on a set of rice cultivars. The first experiment aimed to assess the symptom severity and intra-plant viral accumulation of four viral isolates, each representing one identified genetic group, on three rice cultivars from western Burkina Faso and 2 reference cultivars. Three successive replications were performed. The second experiment aimed to test the pathogenicity of 13 viral isolates (including those used in the first experiment), from the genetic groups identified in western Burkina Faso on one susceptible rice cultivar. The second experiment was all performed at the same time. For both experiments, induced symptoms (estimated with a specific severity scale), as well as intra-plant viral accumulation (estimated though quantitative PCR), were evaluated for each viral isolate. This experimental work evidenced no differences between the three rice cultivars cultivated in Burkina Faso, and no statistical effect of RYMV genetic group on symptom expression and viral load. We found however that RYMV isolates differed from each other and that the three isolates resulting in strongest symptoms and highest viral loads are from the same lineage (S1bzn). For more details, refer to the main documentation file "Data_Experimental_Methodology.pdf".

稻黄斑病毒（RYMV）对非洲水稻种植的实际发展构成了主要威胁。随着气候变化和农业集约化加剧了疫病的风险，迫切需要理解RYMV在局部尺度上的时空动态。本数据集提供了关于2015年至2021年间在布基纳法索西部收集的RYMV分离株致病性的信息。在控制温室条件下（法国蒙彼利埃IRD，2021-2022年）进行了两项实验，旨在表征布基纳法索西部已识别的主要病毒遗传组的病毒分离株的表型，针对一组水稻品种。第一项实验旨在评估四个病毒分离株（每个代表一个已识别的遗传组）在布基纳法索西部三个水稻品种和两个参考品种上的症状严重程度和植物内病毒积累。进行了三次连续重复。第二项实验旨在测试13个病毒分离株（包括在第一项实验中使用的分离株）在布基纳法索西部已识别的遗传组上对一种感病水稻品种的致病性。第二项实验同时进行。对于两项实验，均对每个病毒分离株的诱导症状（使用特定严重程度量表估计）以及植物内病毒积累（通过定量PCR估计）进行了评估。这一实验工作表明，在布基纳法索种植的三个水稻品种之间没有差异，也没有RYMV遗传组对症状表达和病毒载量的统计学影响。然而，我们发现RYMV分离株之间存在差异，导致症状最严重和病毒载量最高的三个分离株来自同一谱系（S1bzn）。欲了解更多详情，请参阅主要文档文件“Data_Experimental_Methodology.pdf”。