Dataset of Escherichia coli DH5α in pure culture on different agar media

<i>Escherichia coli </i>DH5α<i> </i>(ATCC 53868) is the workhorse in molecular cloning. But, how does a pure culture of the bacterium look like on solid agar medium? Answer to this question depends on the medium used. Typically, <i>E. coli</i> DH5α would take on a beige, round colony morphology when grown on rich medium such as LB agar or Tryptic Soy Agar. However, the beige colour is absent from <i>E. coli</i> DH5α colonies grown on minimal salts agar such as a formulated colourless agar used in this study. Pictures of <i>E. coli</i> DH5α in pure culture on different agar media are encapsulated as a zip file dataset with this abstract. Incubation conditions were 37 ^oC under variable length of time on different agar media.

大肠埃希菌（Escherichia coli）DH5α（ATCC 53868）是分子克隆领域的经典宿主菌株。那么，该菌株的纯培养物在固体琼脂培养基上会呈现何种形态？这一问题的答案取决于所使用的培养基类型。通常而言，大肠埃希菌DH5α在LB琼脂（LB Agar）或胰蛋白胨大豆琼脂（Tryptic Soy Agar）等富营养培养基上培养时，会呈现米色圆形菌落形态。然而，在本研究采用的化学合成无色基础无机盐琼脂培养基上培养的大肠埃希菌DH5α菌落，则无米色色泽。本摘要附带的压缩包数据集收录了不同琼脂培养基上大肠埃希菌DH5α纯培养物的相关图像。所有培养均在37℃条件下进行，不同培养基对应的培养时长各不相同。