TCR repertoire analysis reveals increased host CTL diversity induced by CD8a ALN-1

Collectively, these data present evidence that the antitumor response mediated by CD8a ALN-1 correlates with clonal expansion and increased TCR repertoire diversity in the TDLN and the tumor tissue, implying epitope spreading. Treatment of mice with PBS, OVA alone and OVA + CD8a ALN-1. Isolation of TDLN and tumor tissue. FACS of CD8+ T cells. RNA isolation and TCR sequencing analysis.