Additional file 1 of Early-life undernutrition induces enhancer RNA remodeling in mice liver

Additional file 1: Figures S1–S10. Figure S1. Overview of GRO-seq library construction. A, C Isolated liver nuclei stained with DAPI. B, D Amplified DNA library range from 200–500 bp. Figure S2. Agarose gel extraction for cDNA fragment from 150nt-500nt in GRO-seq library construction. Figure S3. Impact of maternal PRD on total transcripts. Differentially expressed genes between the offspring of the dams fed the PRD and NCD at the age of 4 weeks (A) and 7 weeks (B) in RNA-seq were analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG). Fold Change > 1.5, p 1.5, p

附加文件1：图S1至S10。图S1：GRO-seq文库构建概况。A、C：经4',6-二脒基-2-苯基吲哚（DAPI）染色的分离肝细胞核；B、D：扩增得到的DNA文库片段大小范围为200~500 bp。图S2：GRO-seq文库构建过程中150 nt~500 nt cDNA片段的琼脂糖凝胶回收。图S3：母体PRD对总转录本的影响。对饲喂PRD与NCD的母鼠所产子代在4周龄（A）和7周龄（B）时的RNA-seq差异表达基因进行了京都基因与基因组百科全书（KEGG）富集分析。差异倍数>1.5，p 1.5，p