APPENDIX 4 in Fungal / bacterial syntrophy of glycerol utilization
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APPENDIX 4. — Primers used for deletion and complementation. GenesPrimer namesSequencesPa_4_7610 Pa_6_55005’-Pa_4_7610A 5’-Pa_4_7610B mk_4_7610E mk_4_7610F 3’-Pa_4_7610C 3’-Pa_4_7610D 5’-Pa_6_5500A 5’-Pa_6_5500B mk_6_5500E mk_6_5500F 3’-Pa_6_5500Ctcctggtgagctgtatgtaggctggacacg ctatttaacgaccctgccctgaaccgaccaggcagtagagtgaaaaggtcgaaggc gccttcgaccttttcactctactgcctggtcggttcagggcagggtcgttaaatag atcggcccaaacccaaacaaaccatgatgccatcgaactggatctcaacagcggtaag cttaccgctgttgagatccagttcgatggcatcatggtttgtttgggtttgggccgat tatccaacgcagcaaaccgacgaagcatct tgtagcctcgtgtgtgtgtgtgtgtgtgag ctatttaacgaccctgccctgaaccgcaagagtgagagagacagttcagacgacggc gccgtcgtctgaactgtctctctcactcttgcggttcagggcagggtcgttaaatag aaaccagccctctcatgacaagaacccatctcatcgaactggatctcaacagcggtaag cttaccgctgttgagatccagttcgatgagatgggttcttgtcatgagagggctggtttPa_6_5500 complementation3’-Pa_6_5500D 5’-Pa_4_5500A bis 3’-Pa_4_5500D bistgggcttgatatcgtgctatattggcggcc aagatcgacgctagaggtgg tatattggcggccgggttaa
