Sampling localities and Genbank accession numbers from individuals of Cambarellus used in this study.

+Locality number, as depicted in Figure 1.*Type specimens or type localities.**Morphologically identified as C. shufeldtii.***Morphologically identified as C. puer.++Sequence from the study of Buhay et al. 2007, tissue originally from the Carnegie Museum of Natural History.Populations termed as ‘C. sp’ are new proposed taxa, according to phylogenetic structure (see Figure 3).Populations from clade I are included in the lineage of C. zempoalensis, species which has to be re-examined by incorporing C. montezumae lermensis in the analysis.