Biotransformed Bear Bile Powder Ameliorates Diet-induced Nonalcoholic Steatohepatitis in Mice

To evaluate the therapeutic effects and underlying mechanisms of BBBP against NASH There are three sets of samples, namely the Control group, Model group, and BBBPH group (JDH group), with 5 samples in each group.To establish a nonalcoholic steatohepatitis model, mice were fed on a high-fat high- sucrose diet (60% kcal fat, 20% kcal protein, 20% kcal carbohydrates and 0.2% cholesterol, D12492, Research Diets, USA) along with drinking water containing 42 g/L sugar (55% sucrose and 45% fructose by weight) for 20 weeks after which they were sacrificed. After 12 weeks, the high-fat high- sucrose diet-induced nonalcoholic steatohepatitis mice were divided into 2 groups (model and JDH (Biotransformed Bear Bile Powder) group). Then, mice in different groups were orally administered 150 mg/kg Biotransformed Bear Bile Powder (the JDH group, n=14) and saline (the model group) daily, concurrent with the high-fat high- sucrose diet.