TurboID-mediated proximal labeling of Drosophila caspase Drice in adult brains
收藏NIAID Data Ecosystem2026-05-01 收录
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资源简介：
V5-TurboID was knock-in tagged in Drosophila caspase Drice which is expressed in adult brains. Flies were fed with 100 µM biotin for protein biotinylation in two biological replicates. Biotinylated protein-containing lysate extracted from adult brains was subjected to FG-NeutrAvidin beads purification followed by on-beads trypsin digestion. Digested peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.
创建时间：
2024-02-06
