Observational study to visualize and confirm Connexin43 localization at the interface between endothelial cells labeled by Flk1:eGFP expression and pericytes labeled by DsRed expression (Ng2:DsRed/+) within murine embryonic stem cell-derived vessels.

Observational study to visualize and confirm Connexin43 localization at the interface between endothelial cells labeled by Flk1:eGFP expression and pericytes labeled by DsRed expression (Ng2:DsRed/+) within murine embryonic stem cell-derived vessels from an Flk1:eGFP/+; Ng2/Cspg4:DsRed/+ animal (collection after 14 days in culture). Images acquired on a Zeiss LSM 880 point-scanning confocal microscope to detect Cx43 at the pericyte-endothelial cell interface. Volumetric images taken through the z-axis were acquired at 40x. Images in the figure are compressed z-stacks within a selected range, but the raw image files in .czi format are also included.

本研究为一项观察性研究，旨在可视化并确认连接蛋白43（Connexin43）在双转基因小鼠Flk1:eGFP/+; Ng2/Cspg4:DsRed/+的胚胎干细胞来源血管中，由Flk1:eGFP标记的内皮细胞与由DsRed（Ng2:DsRed/+）标记的周细胞之间界面处的定位；该血管样本取自体外培养14天后的上述转基因动物。成像采用蔡司LSM 880点扫描共聚焦显微镜，用于检测周细胞-内皮细胞界面处的Cx43。沿Z轴采集的体积图像使用40倍物镜拍摄。本图中的图像为选定范围内的压缩Z堆叠图像，同时附带了.czi格式的原始图像文件。