Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis

Abstract In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically. Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Ozone gas was applied on only 3 wounds (test group) and the remaining 3 were left to natural healing (control group). Biopsy samples were taken from one of the wounds in each group on the third day, from another wound of each group on the seventh day, and from another one on the tenth day. Routine histological analysis and immuno-histochemical staining were performed to investigate transforming growth factor-beta (TGF-β) and (VEGF) expressions. Results: No statistical difference was found between the test and control groups in terms of collagen fibers, epithelial formation and inflammation scores. A VEGF expression found in the test group was statistically higher than control group samples taken on the 3rd and 7th day. There was no statistical difference between the test and control groups in terms of TGF-β expression on any of the sampling days. Conclusion: The topical application of ozone gas could be effective in the early stages of wound healing by increasing the amount of VEGF expression. Clinical Relevance: Topical application of ozone gas may be effective in the early stages of oral wound healing.

摘要：本研究通过组织学与免疫组织化学手段，评估了臭氧疗法对创面二期愈合的影响。材料与方法：本研究选用8头健康猪，采用打孔法在每头猪的腭侧牙龈上制备6个直径为10 mm的创面。其中3个创面施加臭氧气体（实验组），剩余3个创面任其自然愈合（对照组）。分别于第3天、第7天、第10天从每组各取1个创面活检样本：第3天取每组1个创面样本，第7天取每组另1个创面样本，第10天取每组剩余的1个创面样本。通过常规组织学分析与免疫组织化学染色，检测转化生长因子-β（transforming growth factor-beta, TGF-β）与血管内皮生长因子（vascular endothelial growth factor, VEGF）的表达水平。结果：实验组与对照组在胶原纤维、上皮形成及炎症评分方面均无统计学差异。实验组在第3天和第7天采集的样本中，血管内皮生长因子（VEGF）表达水平显著高于对照组；而在所有采样时间点，两组转化生长因子-β（TGF-β）的表达水平均无统计学差异。结论：局部应用臭氧气体可通过提升血管内皮生长因子（VEGF）的表达水平，在创面愈合早期阶段发挥有益作用。临床相关性：局部应用臭氧气体或许可在口腔创面愈合的早期阶段产生有效治疗效果。