Effect of splenectomy on liver regeneration after 70% resection in a mouse model.

In this work, the effect of splenectomy on liver regeneration was studied. For this purpose in 1 series of experiments the animals were reproduced splenectomy, and after 7 days 70% liver resection was performed. It was found that spleen removal leads to a decrease in the migration of Ly6C+ monocytes into the regenerating liver. Using transcriptional analysis it was shown that spleen and peripheral blood monocytes differ in the expression of genes associated with the synthesis of surface receptors necessary for cell migration. The obtained data explain the reason for the preferential migration of spleen monocytes rather than peripheral blood monocytes into the regenerating liver. A model of resection of 70% of the liver mass was reproduced in mice. 24 hours after resection, blood (n=3) and spleen (n=3) were collected from animals, from which CD115+ monocytes were obtained by magnetic sorting and used in the study. Monocytes from peripheral blood (n=3) and spleen (n=3) of intact animals, obtained by magnetic sorting by CD115 marker, served as control.