Additional file 7 of Minocycline protects against microgliopathy in a Csf1r haplo-insufficient mouse model of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP)

Additional file 7: Figure S1. Identification of CSF1R expression in Csf1r+/— microglia and Csf1r+/− mouse brain. Figure S2. CSF1R haploinsufficiency does not alter the synaptic function in female mouse brain. Figure S3. CSF1R haploinsufficiency results in noteworthy changes in the enrichment gene sets of phagosome or toll-like receptor pathway. Figure S4. CSF1R haploinsufficiency results in enhancement in the mRNA levels of Tnf-α and Il-1β both in vivo and in vitro. Figure S5. CSF1R haploinsufficiency does not affect the phagocytosis of microsphere beads by microglia. Figure S6. Minocycline exposure partially inhibits the mRNA levels of Tnf-α and Il-1β in Csf1r+/— microglia or Csf1r+/— mouse brain. Figure S7. The density of microglia is reduced in Csf1r+/— mouse brain. Figure S8. Astrocyte is activated in Csf1r+/— mouse brain.

补充文件7：图S1。Csf1r+/−小胶质细胞（microglia）与Csf1r+/−小鼠脑内集落刺激因子1受体（CSF1R）的表达鉴定。图S2。CSF1R单倍体不足不会改变雌性小鼠脑内的突触功能。图S3。CSF1R单倍体不足会导致吞噬体（phagosome）或Toll样受体通路的富集基因集出现显著变化。图S4。CSF1R单倍体不足会使体内及体外的肿瘤坏死因子-α（Tnf-α）与白细胞介素-1β（Il-1β）的mRNA水平升高。图S5。CSF1R单倍体不足不会影响小胶质细胞对微球珠的吞噬作用。图S6。米诺环素（minocycline）处理可部分抑制Csf1r+/−小胶质细胞或Csf1r+/−小鼠脑内Tnf-α与Il-1β的mRNA水平。图S7。Csf1r+/−小鼠脑内的小胶质细胞密度降低。图S8。Csf1r+/−小鼠脑内存在星形胶质细胞（astrocyte）激活现象。