Gene expression profile at single cell level of CD45+ cells isolated from twitcher (twi) mouse brain (postnatal day 21)

Gene expression profile at single cell level of CD45+ cells isolated from wildtype (twi littermate) mouse brain (postnatal day 21) to evaluate and compare differences in immune cells at the onset of spontaneous clinical disease in a mouse model of globoid cell leukodystrophy. CD45+ cells were isolated from single cell suspension of hemi-brain tissues by positive selection using flow cytometery本数据集包含分离自野生型（twi小鼠同窝仔鼠）出生后第21天小鼠大脑的CD45阳性细胞（CD45+ cells）的单细胞水平基因表达谱，旨在评估并比较球样细胞脑白质营养不良（globoid cell leukodystrophy）小鼠模型中自发性临床疾病发作阶段的免疫细胞差异。实验通过流式细胞术（flow cytometry）阳性分选法，从半脑组织的单细胞悬液中分离得到上述CD45阳性细胞。