SH1037407.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、交流以及开展基于DNA的物种假说（DNA-based Species Hypotheses，下文简称SH）的相关研究工作。目前，国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，简称ITS）序列，均通过应用一组动态距离阈值（0.5%至3.0%左右）被聚类至近似物种水平。所有物种假说都会被赋予一个唯一且稳定的、以数字对象标识符（Digital Object Identifier，简称DOI）形式命名的标识，其分类学与生态学注释将通过分布式的网页端第三方注释工作进行核验。在充分考量该假说下所有序列的鉴定结果的基础上，每个SH都会被尽可能关联至对应的分类单元名称及其所属分类层级（如门、纲、目等）。随后，研究人员会选取一条自动或手动指定的序列作为每个SH的代表序列。这些代表序列已发布于https://unite.ut.ee/repository.php，可供科研社区用于本地序列相似性搜索、高通量测序分析流程等科研场景。随着公共真菌ITS序列数据量的持续增长，该系统与配套数据集将自动完成更新。