Fluorescent microscopy images of osteoblasts on patterned Ti substrates, () complete micron rough Ti substrates, and () complete nano rough Ti substrates after four hours

Osteoblast alignment was detected on the patterned substrates when compared with the entire micron and nano rough Ti substrates. Among the patterned substrates, osteoblast morphology varied. As the nano region width decreased, cells became more round and less diffuse. Magnification = 10×. Nano patterned regions appear darker.<b>Copyright information:</b>Taken from "Nano rough micron patterned titanium for directing osteoblast morphology and adhesion"International Journal of Nanomedicine 2008;3(2):229-241.Published online Jan 2008PMCID:PMC2527665.© 2008 Puckett and Webster, publisher and licensee Dove Medical Press Ltd.

与整体微米级及纳米级粗糙钛（Ti）基板相比，图案化基板上可观测到成骨细胞的定向排列。在各类图案化基板中，成骨细胞的形态存在差异。随着纳米区域宽度的减小，细胞形态愈发趋于圆形，铺展程度逐渐降低。放大倍数为10×。纳米图案区域颜色更深。
**版权信息：** 本文源自《用于调控成骨细胞形态与黏附的纳米粗糙微米图案化钛》，发表于《国际纳米医学杂志（International Journal of Nanomedicine）》2008年；3(2):229-241，于2008年1月在线发表，PMCID：PMC2527665。©2008 Puckett与Webster，出版及授权方为Dove Medical Press有限公司。