Raw data for rASFV I/II in vivo inoculation experiment in Vietnam (July 2024)

Here, we investigated the pathogenicity of rASFV I/II from Vietnam and the associated pathobiological responses in domestic pigs experimentally inoculated with rASFV I/II. Ten healthy 7-week-old female pigs were intramuscularly inoculated with an rASFV I/II strain. Clinical signs, rectal temperatures, and samples were collected daily, and necropsies were conducted postmortem. Viral DNA was detected using qPCR, and serum samples were analysed via ELISA. All inoculated pigs succumbed to infection within 5–7 days post-inoculation (dpi), with a mean of 5.5±0.7 dpi. High fever (>40.5°C) was observed at an average onset of 2.6±0.8 dpi, and the incubation period averaged 3.0±1.1 dpi. Viral DNA was first detected in blood at 2 dpi, with viral genome copies increasing steadily. Postmortem analysis revealed severe congestive splenomegaly and haemorrhagic lymphadenopathy in all pigs. High viral DNA copy numbers were detected in all organs, particularly the liver, lymph nodes, spleen, lungs, and kidneys.

本研究针对越南来源的重组非洲猪瘟病毒I/II型（recombinant African Swine Fever Virus I/II, rASFV I/II）的致病性，以及实验接种该毒株的家猪的相关病理生物学反应展开探究。选取10只健康的7周龄雌性家猪，经肌肉注射接种rASFV I/II毒株。每日采集受试猪的临床症状、直肠体温及样本，并在其死亡后实施剖检。采用实时荧光定量聚合酶链反应（quantitative real-time polymerase chain reaction, qPCR）检测病毒DNA，并通过酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA）分析血清样本。所有接种受试猪均在接种后5~7天内（dpi）因感染死亡，平均死亡时间为5.5±0.7 dpi。受试猪平均在接种后2.6±0.8 dpi出现高热（体温＞40.5℃），潜伏期平均为3.0±1.1 dpi。接种后2 dpi即可在血液中首次检测到病毒DNA，病毒基因组拷贝数随时间稳步升高。剖检分析显示，所有受试猪均出现严重的充血性脾肿大及出血性淋巴结炎；所有器官中均检测到高拷贝数的病毒DNA，其中以肝脏、淋巴结、脾脏、肺脏及肾脏尤为显著。