Additional file 1: Figure S1. of SiO2 and TiO2 nanoparticles synergistically trigger macrophage inflammatory responses
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IL-1β secretion from bone marrow-derived macrophages (BMDMs) stimulated with various inorganic nanoparticles (NPs). a LPS-primed (black circles) or unprimed (white circles) BMDMs were stimulated with the indicated dose of NPs for 4 h at 37 °C. The amount of IL-1β in culture supernatants was measured by ELISA. b LPS-primed BMDMs were stimulated with the indicated combination of NPs (10 μg/cm3 each) for 4 h at 37 °C. The amount of IL-1β in culture supernatants was measured by ELISA. S.D. was less than 10% of the mean of triplicates (not shown). N.D.; not detected. Similar results were obtained in three independent experiments. Figure S2. Concentration-dependent IL-1β secretion from BMDMs stimulated with SiO2 and/or TiO2 NPs. LPS-primed BMDMs were stimulated with the indicated concentration of SiO2 and/or TiO2 NPs for 4 h at 37 °C. The amount of IL-1β in culture supernatants was measured by ELISA. Data are shown as mean + S.D. N.D.; not detected. *P
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2017-12-18
