Insecticidal proteins and methods for their use

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular， the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus， transformed bacteria， plants， plant cells， plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants， as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling， inhibiting growth or killing Lepidopteran， Coleopteran， Dipteran， fungal， Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

本发明提供了用于防治害虫的组合物与方法。所述方法通过将编码杀虫蛋白的核酸序列转化至生物体中得以实现。具体而言，该核酸序列可用于制备具备杀虫活性的植物与微生物。由此，本发明提供了经转化的细菌、植物、植物细胞、植物组织及种子。本发明的组合物包含细菌来源的杀虫核酸与蛋白。所述序列可用于构建表达载体，以便后续将载体转化至包括植物在内的目标生物体中，同时可作为探针用于分离其他同源（或部分同源）基因。本杀虫蛋白可用于防治、抑制生长或杀灭鳞翅目（Lepidopteran）、鞘翅目（Coleopteran）、双翅目（Dipteran）、真菌类、半翅目（Hemipteran）及线虫类害虫种群，同时可用于制备具备杀虫活性的组合物。