The role of hepatic XBP1 in FXR null mice

X-box binding protein 1 (XBP1) is a key component of the unfolded protein response that is activated in response to ER stress. Farnesoid X receptor (FXR) null (Fxr-/-) mice have elevated bile acid levels and progressive liver injury. Hepatic XBP1 pathway is activated in older Fxr-/- mice. This project is to investigate the role of liver XBP1 in Fxr-/- mice. We generated mice with FXR and liver-specific XBP1 double knockout (DKO, Fxr-/-Xbp1LKO) and Fxr-/-Xbp1fl/fl single knockout (SKO) mice. Liver RNA was extracted from chow-fed male 8-10 weeks old SKO and DKO mice.X盒结合蛋白1（X-box binding protein 1，XBP1）是未折叠蛋白反应的关键组分，该反应可在内质网应激（ER stress）刺激下被激活。法尼醇X受体（Farnesoid X receptor, FXR）敲除（Fxr-/-）小鼠体内胆汁酸水平升高，并伴随进行性肝损伤。老年Fxr-/-小鼠的肝脏XBP1通路会被激活。本研究旨在探究肝脏XBP1在Fxr-/-小鼠中的作用。我们构建了FXR与肝脏特异性XBP1双敲除（DKO, Fxr-/-Xbp1LKO）小鼠，以及Fxr-/-Xbp1fl/fl单敲除（SKO）小鼠。我们从普通饲料喂养的8~10周龄雄性SKO与DKO小鼠体内提取了肝脏RNA。