Assessment of anti-diabetic activity of a novel hydrazine-thiazole derivative: in vitro and in vivo method

Diabetes mellitus is a chronic disease resulting in oxidative stress that promotes tissue damage. The appearance of this disease is highly related to lifestyle and food of the population, being of great interest to search for a dietary supplement that can also act by reducing oxidative alterations. Based on the broad range of biological activity of thiazole derivatives, this work aimed to evaluate the in vitro antioxidant activity of a novel hydrazine-thiazole derivative and studies in vivo. In in vivo experiments, the liver extracts of healthy and diabetic Wistar rats were used, with analysis to determine the enzymatic activity of SOD, CAT, GPx, and GR, and determination of lipid peroxidation. Finally, in the blood of these animals, biochemical parameters were evaluated. Statistical evidence of changes caused in liver enzymes and liquid peroxidation was not detected; however, these parameters were also not changed between control groups with and without diabetes. On the other hand, concerning biochemical parameters, significant differences were detected in uric acid, alkaline phosphatase, ALT, and urea, indicating a possible antioxidant protective role of such substances in the liver and kidney of diabetic animals that could be acting by means other than that commonly reported in the literature.

糖尿病（Diabetes mellitus）是一种可引发氧化应激（oxidative stress）并造成组织损伤的慢性疾病。该疾病的发生与人群的生活方式及饮食结构密切相关，因此寻找可通过减轻氧化损伤发挥作用的膳食补充剂（dietary supplement）具有重要研究价值。基于噻唑类衍生物（thiazole derivatives）广泛的生物活性，本研究旨在评估一种新型肼基噻唑衍生物（hydrazine-thiazole derivative）的体外抗氧化活性，并开展体内实验研究。体内实验中，本研究采用健康大鼠与糖尿病模型Wistar大鼠的肝脏提取物，通过检测超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）及谷胱甘肽还原酶（GR）的酶活性，同时测定了脂质过氧化（lipid peroxidation）水平。此外，本研究还对这些实验动物的血液生化指标进行了分析。统计分析未发现肝脏酶活性及脂质过氧化水平存在显著变化；且无论是否诱导糖尿病，对照组间的上述参数均无明显差异。另一方面，针对血液生化指标，研究人员检测到尿酸（uric acid）、碱性磷酸酶（alkaline phosphatase）、丙氨酸氨基转移酶（ALT）及尿素（urea）水平存在显著差异，这表明该类物质可能对糖尿病实验动物的肝脏与肾脏具有抗氧化保护作用，其作用机制或与现有文献报道的常见途径存在不同。